StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...
Free

Citrobacter freundii - Lab Report Example

Cite this document
Summary
This is a core practice when studying diseases discovered in a patient, to know the causative agents and possible treatments. Intuitively, this…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER93.6% of users find it useful
Citrobacter freundii
Read Text Preview

Extract of sample "Citrobacter freundii"

PURPOSE MENT In a microbiology lab, one of the most important activities carried out is determination of the identity of unknown microorganism. This is a core practice when studying diseases discovered in a patient, to know the causative agents and possible treatments. Intuitively, this study makes use of the knowledge acquired from microbiology laboratory sessions to establish the identity of unknown bacterium #20.MATERIALS AND METHODSUnknown #20 was obtained from the instructor. The procedure followed was as stated in Bergey’s manual.

The unknown was streak out using the T streak method, in the presence of Trypticase Soy Agar plate. The plates were placed in an incubator and grown while observing the morphology. A Gram stain was performed on the unknown alongside quality control bacteria. The gram reaction was observed and recorded and specific biochemical tests performed. Given the reaction gave out a gram negative rod, the first test performed was oxidase test. Other tests performed are listed in the table found in the results section.

Biochemical test performedGram stainOxidase testBCP lactoseIndole testCitrate testMotility testRESULTSThe following tablet lists all the biochemical tests carried on the unknown microorganism:TESTPURPOSEREAGENTSOBSERVATIONSRESULTSGram stainTo establish reaction of the microorganism on the gram stainAlcohol, iodine, crystal violet, safraninpink rodsGram negative rodsOxidase testTo establish the presence of cytochrome cOxidase paperBlack color changePositive oxidase testBCP lactoseTo study the fermenting effect of a bacterium on a given carbohydrateNonePurple to yellow color changePositive lactose fermentedIndole TestTo study if an organism can split indole from trytophaneMixture of kovac’s and 1 ml tryptone brothRed ring witnessed at top of brothnegative indole testCitrate testTo determine the ability of organism to utilize citrate as a the sole means for carbon and energySimmons Citrate agarColor change to bluePositive citrate testMotility testTo determine the motility of an organism by means of flagellaSemi-solid medium containing tetrazolium chloride indicatorFuzzy pink color observed from the stab linePositive motility testFigure showing flowchart to determine the identity of the organism:Unknown #20Gram stainGram negative rodOxidase test (positive) Positive negativeCitrobacter freundii Proteus vulgarisEnterobacter aerogenes Proteus mirabilisEscherichia coli Serratia marcescensKlebsiella oxytoca Indole test (positive)(Positive) (negative)Escherichia coli Citrobacter freundiiKlebsiella oxytoca Enterobacter aerogenes Klebsiella pneumonia Citrate test (negative)(Positive) (negative)Citrobacter freundii Klebsiella pneumoniaEnterobacter aerogenesMotility (negative) (Positive) (negative)Citrobacter freundii Enterobacter aerogenesHence unknown #20 is Citrobacter freundiiDISCUSSION AND CONCLUSION After all the differential tests were performed, the results showed that the identity of unknown microorganism was citrobacter freundii.

The results were compared against the class chart showing the classifications of the microorganisms. The determination that the unknown organism was gram negative rod initiated use of TSA slant to inoculate the other tests, Oxidase test, BCP lactose, Indole test, Citrate test, Motility test (Fraser, 76). All the results were well worked out for the the tests except for the citrate test which gave a false negative results. The error was noted given that, for this test, the result was inconsistent with other tests.

The test was repeated and was able to give a positive result. In conclusion, the unknown organism was found to be Citrobacter freundii, following a series of eliminations made from the tests. Citrobacter freundii is in the Enterobacteriaceae family and are gram-negative bacilli (Fraser p. 20). It is normally found in sewage and intestinal tract of animals and humans. It can cause disease to its host. Work cited Brenner, Don J, Noel R. Krieg, and James T. Staley. Bergeys Manual of Systematic Bacteriology: Volume Two, Part a.

New York: Springer, 2005. Internet resource.Fraser, Susan L. Enterobacter Infections in Medscape Reference. New York: Willey, 2012. Retrieved from: http://emedicine.medscape.com/article/216845-overview#aw2aab6b2b2

Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Citrobacter freundii Lab Report Example | Topics and Well Written Essays - 250 words”, n.d.)
Retrieved de https://studentshare.org/medical-science/1660438-citrobacter-freundii
(Citrobacter Freundii Lab Report Example | Topics and Well Written Essays - 250 Words)
https://studentshare.org/medical-science/1660438-citrobacter-freundii.
“Citrobacter Freundii Lab Report Example | Topics and Well Written Essays - 250 Words”, n.d. https://studentshare.org/medical-science/1660438-citrobacter-freundii.
  • Cited: 0 times

CHECK THESE SAMPLES OF Citrobacter freundii

Quality of Drinking Water and Climatic Changes

The paper "Quality of Drinking Water and Climatic Changes" discusses the significant level of difference between total plate counts found in drinking water during warmer periods.... The presence of E.... coli in turn means that fecal bacteria are infecting the drinking water… Heavy efforts are required to spread awareness about the risks of contamination in drinking water that is being used regularly by households and preventive measures that can defend them against these threats....
10 Pages (2500 words) Essay

Environmental engineering

It uses microbial technology under different environmental conditions in the overall process (Metcalf and Eddy, 2003). BNR process has two states 1) Nitrification – The… version (oxidation) of ammonia to nitrate caused by bacteria in the presence of oxygen and 2) Denitrification - The conversion (reduction) of nitrate to nitrogen gas caused by the absence of oxygen....
2 Pages (500 words) Essay

Trophic Levels and Organism Localization and Interaction Specific to the Philippines

The region boasts of fish and corals as compared to other marine environment on the earth (Unico Conservation Foundation, 2012).... The reefs in this region also have a higher… The region also boasts of a higher number of whale shark, hundred of species of corals.... The significance of coral reefs is best seen in the coastline (Unico Conservation Foundation, 2012)....
3 Pages (750 words) Research Paper

Cell Structure (Bio slp Mod 1)

Consequently living things can be broadly classified into prokaryotes or eukaryotes depending on their cell structure.... These two groups differ by their subcellular structure where eukaryotes have membrane… Eukaryotic cell, therefore, contain complex structures formed by internal membranes and cytoskeleton whereas, prokaryotic cells are simple with less complex structured organelles....
2 Pages (500 words) Essay

The Discovery of Antibiotics

As much as this is true, what remains a real wonder is the continued increase of antibiotic resistance in communities, health centers, as well as the environment affiliated to their… The behavior shown by people in overusing antibiotics gives room to enhancing the extraordinary ability of microbes to take advantage of each available source of resistance genes besides each way of horizontal gene transfer in the process of developing various ways of The likely development of resistance or tolerance compromises the successful application of therapeutic from the first day of usage....
8 Pages (2000 words) Scholarship Essay

Enterotube case study

Many members of this family compose… However, many members such as Enterobacter, Shigella, citrobacter etc.... The enterobactericeae family consists of gram negative, facultative gram negative and rod shaped bacteria that are incapable of forming endospores.... are also known to cause harmful diseases as well. A 12 year old boy is admitted in the ER....
4 Pages (1000 words) Lab Report

Enterobacteriaceae - Laboratory Tests to Identify Differences

This paper "Enterobacteriaceae - Laboratory Tests to Identify Differences" focuses on the group of microorganisms that are causal agents in various diseases, notably intestinal ones.... The isolation of strains is difficult because these bacteria share so many similar characteristics.... nbsp;… Experts have suggested different media in order to isolate specific strains of bacteria such as salmonella, among others....
20 Pages (5000 words) Research Paper

Determining If the Organism Could Produce Acid

The flow chartThis can be presented a flow chart as followsThe unknown AGram stainGram-negative rodOxidase positive testsNegative positiveProteus Vulgaris Pseudomonas aureomycinMorganella morganii citrobacter freundiiIndole testCitrate testMethyl red test DiscussionAfter several tests were conducted, the results indicated that the unknown marked A was a gram-negative rod....
6 Pages (1500 words) Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Contact Us